Safranin dye

Medical device. IVDD 98/79 / CE

SPECIFIC USE AND DESCRIPTION

The stains used in Bacteriology are used to help the morphological visualization of bacteria, which due to their insignificant size are practically transparent. Although it is not its main application, the simple coloring with Safranina facilitates the observation of germs, which stand out for their pink color. It is used mainly as a contrast dye in Gram staining, replacing the diluted fuchsin that was used

above, since it better differentiates Gram negatives. It can also be used as in simple dyes, although it is usually preferred to other dyes that give a more pronounced hue. It is advisable to carry out the staining from young cultures (24-36 h).

Exclusive use as laboratory reagent Supplied with explanatory leaflet of the instructions for use. Presented in opaque amber PET bottles with a white sealed cap.

SIMPLE COLORING WITH SAFRANINE

Method:

Take a clean and degreased slide. (eg codes B100001, etc. general cat.).

Make a fine and homogeneous spread. It can be made, directly from a broth culture, from a bacterial suspension obtained by homogenizing 1-2 colonies in saline solution, or from a direct sample from a patient.

Let dry, and fix the extension by passing the slide over the flame of a bunsen burner. Avoid overheating.

Place the slide on a staining rack (eg codes S002, S003 etc. cat general).

Stain the smear by flooding the slide with SAFRANINE, for 2 minutes.

Wash with water using a washing bottle (eg codes 443000 and 191638 cat.

general) until the washing liquid is no longer purple.

Let it drain vertically (see drippers codes CP24 and CP30) and dry at room temperature.

Visualize with an immersion objective, placing a drop of oil between the smear and the lens.

Reading :

Bacteria and other elements appear pink.

Storage:

We recommend keeping the product between 15 and 25ºC until the expiration date indicated on the label.

Expiration:

Two years from the date of preparation.

Our microbiology stains are based on the American Society for Microbiology formulas. The quality assurance system adopted complies with the requirements of the ISO9001: 2000 standard "Quality systems.

Model for quality assurance in production, installation and after-sales service ». Upon request, a copy of the ISO 9001: 2000 certificate can be supplied.

See also the SAFETY DATA SHEET corresponding to this product.

a) Reference 805050:

• Hazard: h426
• Transport regulations: UN 1993. ADR: 3 / III. IMDG: 3 / III IATA: 3 / III

b) Reference 805150:

Minimum unit of sale: 1 bottle or kit. Consult with the commercial department the special transport conditions.