Error message
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Ziehl differential staining
Kit 805010 with 4 bottles of 250ml:
1 fuchsin (805020)
1 methylene blue (805030)
2 Ziehl bleach (805011)
SPECIFIC USE AND DESCRIPTION
The stains used in Bacteriology are used to help the morphological visualization of bacteria, which due to their insignificant size are practically transparent.
Differential methods make it possible to distinguish bacteria belonging to different staining groups. The Ziehl-Neelsen method differentiates acid-resistant bacteria (ABB) from those that are not.
Exclusive use as a laboratory reagent. It is supplied with an explanatory leaflet of the instructions for use. Presented in bottles made of amber PET and with a white sealing cap.
DIFFERENTIAL COLORATION BY THE ZIEHL-NEELSEN METHOD
Method:
Take a clean and degreased slide (eg B100004, etc, general catalog)
Make a fine and homogeneous spread. It can be made, directly from a broth culture, from a bacterial suspension obtained by homogenizing 1-2 colonies in saline solution, or from a direct sample from a patient.
Let dry, and fix the extension by passing the slide over the flame of a bunsen burner. Avoid overheating.
Place the slide on a staining rack (eg code S003, etc., general cat.)
Place a piece of filter paper, smaller than the size of the slide, on top of it, so that it covers the preparation.
Soak it in basic carbol fuchsin.
Heat slightly below the slide until white smoke is released (avoid boiling).
Repeat the heating operation 2-3 times, adding fuchsin on the filter paper, if it has dried. Let cool.
Wash with water with the help of a washing bottle (eg code 191638 cat general) making sure that the filter paper is washed away.
Bleach by flooding with Ebner's alcohol. Immediately wash off with water.
Contrast staining by flooding the slide with methylene blue for 2 minutes. Wash with water, until the washing liquid has no blue color. Let drain, and dry at room temperature. Visualize with an immersion objective, placing a drop of oil between the smear and the lens.
Reading:
The acid-fast bacilli are stained red and the rest of the elements blue.
Storage:
We recommend keeping the product between 15 and 25ºC until the expiration date indicated on the label.
Expiration:
Two years from the date of preparation.
Our microbiology stains are based on the American Society for Microbiology formulas.
The quality assurance system adopted complies with the requirements of the ISO 9001: 2000 standard “Quality systems. Model for quality assurance in production, installation and after-sales service ». By request
A copy of the ISO 9001 certificate can be supplied.
See also the SAFETY DATA SHEET corresponding to this product.
Minimum unit of sale: 1 bottle or kit. Consult with the commercial department the special transport conditions.
